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KMID : 1098420070150010001
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Korean Journal of Medicinal Crop Science
2007 Volume.15 No. 1 p.1 ~ p.5
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Development of SCAR Marker for Discriminating between Violet Flowered Lines and White Flowered Lines in Chinese Bellflower (Platycodon grandiflorum A.)
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Pack Chun-Geon
Lee Sang-Chul
Sung Jung-Sook
Seong Nak-Sul
Bang Kyong-Hwan
Kim Ok-Tae
Kim Dong-Soon
Kim Dong-Hwi
Park Hee-Woon
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Abstract
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To develop a convenient method for discriminating between violet flowered lines and white flowered lines in Chinese bellflower, RAPD analysis was carried out and SCAR markers were generated. Eighteen specific RAPD bands were obtained from 6 OPERON primer sets. Two out of eighteen RAPD bands were cloned into pGEM-T-Easy vectors and then subjected to the nucleotide sequence analysis. PgR1 and PgR2 DNA fragment, each specific for violet and white flowered lines, consist of 887 bp and 863 bp sequences, respectively. Two SCAR markers were developed from RAPD clones: SPgR1 (355 bp) from PgR1 and SPgR2 (493 bp) from PgR2. One (SPgR2) of these two markers was useful to differentiate between violet flowered lines and white flowered lines in Chinese bellflower.
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KEYWORD
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Platycodon grandiflorum, white flowered lines, violet flowered lines, RAPD, SCAR marker, discrimination
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